jc 1 mitomp detection kit (Dojindo Labs)
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Dojindo Labs
jc 1 mitomp detection kit
Jc 1 Mitomp Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/JC-1+MitoMP+Detection+Kit/pmc13626044-424-1-8
Average 96 stars, based on 232 article reviews
Jc 1 Mitomp Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/JC-1+MitoMP+Detection+Kit/pmc13626044-424-1-8
Average 96 stars, based on 232 article reviews
jc 1 mitomp detection kit - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination"
Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination
Journal: Science Advances
doi: 10.1126/sciadv.aee4577
Figure Legend Snippet: ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Techniques Used: Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Immunofluorescence, Western Blot, Analysis, Activity Assay, Expressing, Immune Cell, Two Tailed Test
Figure Legend Snippet: ( A ) Fluorescence images of ZHDG internalization in MFC cells. ( B ) Quantification of the intracellular ZHDG fluorescence intensity. ( C ) Viability of MFC cells across treatment groups. ( D ) Annexin V–FITC/PI staining (fluorescence and flow cytometry). ( E ) TEM images of ultrastructural damage in MFC cells. ( F ) Relative LDH release. ( G ) Intracellular ATP levels. ( H ) Immunofluorescence analysis of CRT exposure and HMGB1 release. ( I ) Zinpyr-1 staining in different treatment groups. ( J ) Quantification of JC-1 aggregates and monomers. ( K ) JC-1 staining (flow cytometry). ( L ) Extracellular dsDNA release. ( M ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and AIM2. ( N ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( O ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( P ) Western blot analysis of PANoptosis-related proteins in si AIM2 MFC cells treated with ZHDG + NIR. ( Q ) Schematic of ZHDG under NIR photoactivation inducing AIM2-PANoptosome formation and ICD. Data in (B), (C), (F), (G), and (L) are mean ± SD. Statistical analysis for (B), (C), (F), (G), and (L) was performed using a two-tailed unpaired Student’s t test. (Q) was created in BioRender. Fei, G. (2026) https://BioRender.com/wcbo7ma .
Techniques Used: Fluorescence, Staining, Flow Cytometry, Immunofluorescence, Analysis, Western Blot, Two Tailed Test
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